qPCR定量分析微RNA和DNA甲基化状态][Quantification of microRNAs and DNA methylation status by qPCR]

qPCR定量分析微RNA和DNA甲基化状态 Quantification of microRNAs and DNA methylation status by qPCR


课程网址:	http://videolectures.net/nib_redshaw_methylation_status/
主讲教师:	Nicholas Redshaw
开课单位:	无
开课时间:	2015-06-01
课程语种:	英语
中文简介:	microRNA和DNA甲基化都是控制基因表达的重要表观遗传学机制。最近，人们对这两个领域的兴趣激增，导致了分析短RNA和甲基化状态的创新策略的发展，qPCR被确立为定量的主要方法。本讲座描述和评估了目前可用的通过qPCR定量微RNA和DNA甲基化的方法，并强调了为了获得最佳数据必须考虑哪些因素。
课程简介:	MicroRNAs and DNA methylation are both critical epigenetic mechanisms for the control of gene expression. The recent explosion of interest in these two areas has led to the development of innovative strategies for the analysis of short RNAs and methylation status, with qPCR established as a leading method for quantification. This talk describes and evaluates the currently available methods used for the quantification of microRNAs and DNA methylation by qPCR and highlights what factors must be taken into consideration in order to achieve optimal data.
关键词:	microRNA; DNA甲基化; 遗传学
课程来源:	视频讲座网
数据采集:	2021-12-14：nkq
最后编审:	2021-12-14：nkq
阅读次数:	55

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