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金属表面纳米结构蛋白质单分子层生化活性的验证Verification of Biochemical Activity for Protein Monolayers Nanostructured on Gold Surfaces |
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| 课程网址: | http://videolectures.net/slonano07_scoles_vba/ |
| 主讲教师: | Giacinto Scoles |
| 开课单位: | 普林斯顿大学 |
| 开课时间: | 2008-02-12 |
| 课程语种: | 英语 |
| 中文简介: |  |
| 课程简介: | We demonstrate that an Atomic Force Microscope (AFM) can be used to immobilize a di-cysteine-terminated protein (Maltose Binding Protein, MBP-cys-cys for short) at well-defined locations directly on gold substrates via nanografting, and characterize the in situ bioactivity of these proteins within the fabricated nanopatterns. This method exploits the high spatial and orientational control of protein monolayer assembly allowed by nanografting, combined with the high sensitivity of the AFM for detecting ligand-binding events. The maltose-mediated conformational changes within the MBP have been found to change the AFM-tip-protein interaction, therefore causing the frictional signal to change. Our data show that the protein ligand-binding function is maintained upon the immobilization. |
| 关 键 词: | 纳米移植; 生物活性; 配体结合 |
| 课程来源: | 视频讲座网 |
| 最后编审: | 2019-09-21：cwx |
| 阅读次数: | 45 |