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用于局部益生菌递送基于藻酸盐的微胶囊：评估细菌的截留，释放和生长Alginate based microcapsules for local probiotic delivery: Evaluation of bacterial entrapment, release, and growth |
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| 课程网址: | http://videolectures.net/biophysics2018_mirtic_bacterial_entrapme... |
| 主讲教师: | Janja Mirtič |
| 开课单位: | 卢布尔雅那大学 |
| 开课时间: | 2018-07-09 |
| 课程语种: | 英语 |
| 中文简介: |  将益生菌递送至人体不同的作用部位可以帮助预防甚至治疗多种疾病。益生素的作用要么基于局部微生物群的形成，要么与宿主细胞相互作用，从而导致疾病进展的逆转。这项研究的主要目的是开发用于递送益生菌的微胶囊，以促进其延长的生存期以及有效的复兴以及目标表面的成功定殖。包封是基于藻酸盐与钙离子的交联，并通过使用膜振动技术将补充了益生菌的聚合物分散体造粒，然后通过聚电解质络合进行脱乙酰壳多糖包衣来进行。微囊通过冻干进一步干燥。最多包封了2×10 10 CFU / g益生菌孢子干微囊，实现了100％掺入可复活益生菌的微囊。壳聚糖涂层改善了它们的生物粘附潜力，但对细菌的生存力和生长动力学没有负面影响。海藻酸盐基质足以捕获细菌，允许营养物和氧气向内扩散，并且只需解开缠结即可使细胞快速释放。出乎意料的是，已经是纳米的壳聚糖薄层代表了一个屏障，这也使益生菌得以持续释放。收集的结果表明，研制的载有益生菌的微胶囊适合局部递送至牙周袋中（1）。 |
| 课程简介: | The delivery of probiotics to different sites of action within the human body can help to prevent or even treat several diseases. The action of priobiotics is either based on the shaping of local microbiota or interacting with host cells resulting in the reversal of disease progression. The primary aim of this study was to develop microcapsules for delivery of probiotic bacteria that promote their prolonged survival and efficient revival and successful colonisation of the target surface. Encapsulation was based on alginate crosslinking with calcium ions and was performed by prilling the polymer dispersion supplemented with the probiotic using membrane vibration technology, followed by chitosan coating by polyelectrolyte complexation. The microcapsules were further dried by lyophilisation. Up to 2 ×10 10 CFU/g dry microcapsules of probiotic spore was encapsulated, achieving 100% of microcapsules with incorporated revivable probiotics. The chitosan coating improved their bioadhesion potential, but did not have negative impact on viability and growth kinetics of the bacteria. Alginate matrix was sufficient for bacteria entrapment, allowed the inward diffusion of nutrients and oxygen, and was simply disentangled to enable fast cell release. Surprisingly, already nanometer thin chitosan layer, represented a barrier, which enabled also sustained release of the probiotic bacteria. The collected results propose that the developed probiotic-loaded microcapsules are suitable for local delivery into periodontal pockets ( 1 ). |
| 关 键 词: | 益生菌; 微胶囊; 膜振动技术 |
| 课程来源: | 视频讲座网 |
| 数据采集: | 2020-11-22：cjy |
| 最后编审: | 2020-11-22：cjy |
| 阅读次数: | 41 |