图 书 馆
利用脂质结合毒素成像脂质Imaging lipids using lipid-binding toxins |
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| 课程网址: | hthttp://videolectures.net/preglov_kobayashu_lipid_binding_toxins... |
| 主讲教师: | Toshihide Kobayashi |
| 开课单位: | 国家科学研究中心 |
| 开课时间: | 2018-09-28 |
| 课程语种: | 英语 |
| 中文简介: |  生物膜表现出特定的脂质组织，例如跨双层不对称性和结构域结构。然而，脂质的详细组织还不是很清楚，主要是因为缺乏对脂质进行成像的特定和可靠的工具。可视化内源性脂质的一种策略是标记它们。报道了几种脂质特异性抗体。然而，很少有蛋白质被报道与特定的脂质结合。 脂质的特征之一是其在动物界中的多样性。各种生物体已经开发出蛋白质和肽毒素，它们与生产细胞不包含的特定脂质强烈结合。这些毒素的无毒衍生物最近成为对脂质成像的有用和强大的工具。在本次讨论会中，我将总结我们使用脂质结合毒素结合各种先进的显微镜技术对脂质，尤其是脂筏（即鞘脂胆固醇复合物）进行成像的尝试。这些方法提供了对不同病理生理条件下脂质的组织和动力学的深入了解。 |
| 课程简介: | Biomembranes exhibit specific lipid organization, such as transbilayer asymmetry and domain structures. However, detailed organization of lipids is not well understood mainly because of the lack of specific and reliable tools to image lipids. One strategy to visualize endogenous lipids is to label them. Several lipid-specific antibodies are reported. However, very few proteins have been reported that bind specific lipids. One of the characteristic features of lipids is its diversity among animal kingdoms. Various organisms have developed protein and peptide toxins that strongly bind specific lipids that the producing cells do not contain. Non-toxic derivatives of these toxins recently become useful and powerful tools to image lipids. In this Colloquium, I will summarize our attempt to image lipids, especially lipid rafts (i.e. sphingolipid-cholesterol complex), using lipid-binding toxins in combination with various advanced microscope techniques. Such approaches provide insights into the organization and the dynamics of lipids in various pathophysiological conditions. |
| 关 键 词: | 生物膜; 可视化内源性脂质; 脂质特异性抗体 |
| 课程来源: | 视频讲座网 |
| 数据采集: | 2021-08-12：nkq |
| 最后编审: | 2021-08-28：nkq |
| 阅读次数: | 28 |