图 书 馆
Nep1样蛋白的膜相互作用机制Mechanism of membrane interactions of Nep1-like proteins |
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| 课程网址: | http://videolectures.net/biophysics2018_anderluh_membrane_interac... |
| 主讲教师: | Gregor Anderluh |
| 开课单位: | 卢布尔雅那化学研究所 |
| 开课时间: | 2018-07-09 |
| 课程语种: | 英语 |
| 中文简介: |  |
| 课程简介: | Necrosis and ethylene-inducing peptide 1 (Nep1)-like proteins (NLPs) are large protein family of virulence factors that are secreted by various plant pathogens and can be found in bacteria, fungi and oomycetes. Upon membrane interactions they are able to trigger plant tissue necrosis and immunity-associated responses in various dicotyledonous plants. The molecular details of their cytolytic mechanism are not known in a great detail. Recently we have identified glycosyl inositol phospho ceramides (GIPC), plant-related sphingolipids, as a target molecule for NLPs binding to plant membranes. Surface plasmon resonance showed that NLP proteins can bind sugars glucosamine and mannosamine, which are often present as the terminal sugars in GIPCs, by millimolar affinity. Structural analysis of NLP protein from oomycete Pythium aphanidermatum in complex with the sugar have indicated structural changes in the protein induced by sugar binding and allow to elucidate the importance of the metal ion coordination for binding. Furthermore, the structure of the non-toxic representative of NLPs, HaNLP3, from oomycete Hyaloperonospora arabidopsidis indicated differences between the proteins that are important for the cytotoxicity. Collectively, we have provided important molecular insights into the mechanisms of cytolytic activity of toxic NLP proteins towards plant plasma membranes. |
| 关 键 词: | 坏死和乙烯诱导肽1（Nep1）样蛋白（NLPs）; 金属离子配位对结合; 蛋白质发生结构变化 |
| 课程来源: | 视频讲座网 |
| 数据采集: | 2021-11-05：zkj |
| 最后编审: | 2021-11-05：zkj |
| 阅读次数: | 120 |