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单价T细胞抗原受体复合物驱动T细胞抗原识别Monovalent T-cell antigen receptor complexes drive T-cell antigen recognition |
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| 课程网址: | http://videolectures.net/biophysics2018_brameshuber_antigen_recog... |
| 主讲教师: | Mario Brameshuber |
| 开课单位: | 应用物理研究所 |
| 开课时间: | 2018-07-09 |
| 课程语种: | 英语 |
| 中文简介: |  |
| 课程简介: | T-cells recognize MHC-embedded antigenic peptide fragments (pMHCs) on antigen presenting cells (APCs) through their clonotypic T-cell antigen receptors (TCRs), which typically feature only moderate affinities towards their pMHC ligands. Higher order TCR-structures, either constitutively present or dynamically formed upon pMHC binding, have thus long been considered instrumental for intracellular T-cell signaling and for maintaining high antigen sensitivity. Here we have determined the stoichiometry of the TCR and their non-covalently associated CD3 signaling chains in living antigen-experienced T-cells with the use of (i) single molecule Fluorescence Recovery After Photobleaching, (ii) Förster Resonance Energy Transfer (FRET) and (iii) photon arrival time - based fluorescence correlation spectroscopy (FCS) measurements. Using these approaches, we find exclusively monomeric TCR entities prior to and during pMHC binding. Hence, molecular events other than TCR clustering drive the recognition pMHCs at both high and low densities. |
| 关 键 词: | T细胞; 单体TCR实体; 克隆型T细胞 |
| 课程来源: | 视频讲座网 |
| 数据采集: | 2021-11-12：zkj |
| 最后编审: | 2021-11-12：zkj |
| 阅读次数: | 39 |