图 书 馆
用于局部益生菌递送的海藻酸钠微胶囊：细菌包埋、释放和生长的评价Alginate based microcapsules for local probiotic delivery: Evaluation of bacterial entrapment, release, and growth |
|
| 课程网址: | http://videolectures.net/biophysics2018_mirtic_bacterial_entrapme... |
| 主讲教师: | Janja Mirtič |
| 开课单位: | 卢布尔雅那大学 |
| 开课时间: | 2018-07-09 |
| 课程语种: | 英语 |
| 中文简介: |  |
| 课程简介: | The delivery of probiotics to different sites of action within the human body can help to prevent or even treat several diseases. The action of priobiotics is either based on the shaping of local microbiota or interacting with host cells resulting in the reversal of disease progression. The primary aim of this study was to develop microcapsules for delivery of probiotic bacteria that promote their prolonged survival and efficient revival and successful colonisation of the target surface. Encapsulation was based on alginate crosslinking with calcium ions and was performed by prilling the polymer dispersion supplemented with the probiotic using membrane vibration technology, followed by chitosan coating by polyelectrolyte complexation. The microcapsules were further dried by lyophilisation. Up to 2 ×10 10 CFU/g dry microcapsules of probiotic spore was encapsulated, achieving 100% of microcapsules with incorporated revivable probiotics. The chitosan coating improved their bioadhesion potential, but did not have negative impact on viability and growth kinetics of the bacteria. Alginate matrix was sufficient for bacteria entrapment, allowed the inward diffusion of nutrients and oxygen, and was simply disentangled to enable fast cell release. Surprisingly, already nanometer thin chitosan layer, represented a barrier, which enabled also sustained release of the probiotic bacteria. The collected results propose that the developed probiotic-loaded microcapsules are suitable for local delivery into periodontal pockets ( 1 ). |
| 关 键 词: | 细菌包埋; 生长; 释放 |
| 课程来源: | 视频讲座网 |
| 数据采集: | 2020-11-29：yxd |
| 最后编审: | 2020-11-29：yxd |
| 阅读次数: | 61 |