第四讲-基因工程(续)Lecture 4 - Genetic Engineering (cont.) |
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课程网址: | http://videolectures.net/yalebeng100s08_saltzman_lec04/ |
主讲教师: | W. Mark Saltzman |
开课单位: | 耶鲁大学 |
开课时间: | 2011-01-04 |
课程语种: | 英语 |
中文简介: | Saltzman教授继续他关于DNA技术的演讲,讨论了使用两种RNA沉默方法控制基因表达:反义疗法和RNA干扰。讨论了利用质粒、限制性内切酶、连接酶和细菌中的抗生素选择大规模生产蛋白质的分子克隆技术。还描述了聚合酶链反应的步骤和涉及的分子。Saltzman教授解释了如何通过凝胶电泳和Southern杂交检测基因组DNA的突变,例如镰状细胞贫血患者。最后,他举了一个例子,通过将带有lac启动子的胰岛素基因克隆到大型动物的基因组中来诱导和控制胰岛素的表达。 |
课程简介: | Professor Saltzman continues his presentation on DNA technology by discussing control of gene expression using two methods of RNA silencing: anti-sense therapy and RNA interference. Molecular cloning techniques to mass-produce proteins using plasmid, restriction enzymes, ligase, and antibiotic selection in bacteria are discussed. Steps and molecules involved in polymerase chain reaction are also described. Professor Saltzman explains how to detect mutations in genomic DNA, such as in sickle cell anemia patients, by gel electrophoresis and Southern blotting. Finally, he gives an example of inducing and controlling insulin expression in large animals by cloning into their genome the insulin gene with the lac promoter. |
关 键 词: | DNA技术; 控制基因表达; 反义疗法 |
课程来源: | 视频讲座网 |
数据采集: | 2021-11-27:zkj |
最后编审: | 2021-11-27:zkj |
阅读次数: | 102 |