图 书 馆
系统生物学代谢工程作物富集β-胡萝卜素含量的方法Approaches of Systems Biology of Metabolically Engineered Crops Enriched in β-Carotene Content |
|
| 课程网址: | http://videolectures.net/nib_diretto_engineered_crops/ |
| 主讲教师: | Gianfranco Diretto |
| 开课单位: | 意大利国家新技术，能源与可持续经济发展国家机构 |
| 开课时间: | 2013-05-23 |
| 课程语种: | 英语 |
| 中文简介: |  |
| 课程简介: | Carotenoids are secondary metabolites involved, in animals, in the prevention of several animal diseases including cancers and cardio-vascular pathologies. In plants, they play essential functions as photosynthetic pigments in leaves, secondary metabolites in fruits and flowers, and hormone precursors; three out of them (α-/β-carotene, β-crypthoxanthin) cover a fundamental role in human nutrition as precursors of vitamin A. During last 20 years, we have generated a large collection of tomato and potato transgenics enriched in β-carotene and total carotenoids; within them, we have focused our attention on “golden” genotypes, including potato tubers expressing simultaneously three genes of bacterial origin (CrtB, phytoene synthase; CrtI, phytoene desaturase; CrtY, lycopene β-cyclase), and tomato transgenic fruits overexpressing lycopene beta-cyclase (LCY-b), beta-carotene hydroxylase (CHY) or both transgenes. We have performed a global profiling at transcriptional (microarray, next-gen sequencing), metabolomic (GC-/LC-MS) and phenomic level on all these materials. Integration of Gene expression profiles, integrated and high-throughput metabolomics, revealed unscheduled transcript-metabolite correlations and shed light on novel co-regulatory dynamics which emerged in berry ripening or tuber aging during post-harvest storage. A strong influence of carotenoid accumulation on fruit ripening kinetics was observed, probably mediated by the hormones ABA and ethylene, while GO enrichment analysis allowed identification of gene classes specifically regulated in "Golden" fruits. Networks of biological interactions and correlations have been employed to visualize and predict the consequences of metabolic engineering approaches on the whole plant metabolism. Overall, these analyses revealed the central role of carotenoids in regulating both fruit and tuber development and large-scale network analysis proved to be a valuable approach for rational design of new biofortified crops by through identification of higher correlative power nodes (hubs of the network), as potential targets in future breeding programs. |
| 关 键 词: | 生物学; 农业; 类胡萝卜素 |
| 课程来源: | 视频讲座网 |
| 最后编审: | 2020-12-29：yumf |
| 阅读次数: | 49 |