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7.13 微生物遗传学实验7.13 Experimental Microbial Genetics |
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| 课程网址: | http://ocw.mit.edu/courses/biology/7-13-experimental-microbial-ge... |
| 主讲教师: | Prof. Dianne Newman ; Dr. Janis Melvold ; Dr. Laura Croal ; Prof. Michael Laub |
| 开课单位: | 麻省理工学院 |
| 开课时间: | 信息不详。欢迎您在右侧留言补充。 |
| 课程语种: | 英语 |
| 中文简介: |  |
| 课程简介: | In this class, students engage in independent research projects to probe various aspects of the physiology of the bacterium Pseudomonas aeruginosa PA14, an opportunistic pathogen isolated from the lungs of cystic fibrosis patients. Students use molecular genetics to examine survival in stationary phase, antibiotic resistance, phase variation, toxin production, and secondary metabolite production. Projects aim to discover the molecular basis for these processes using both classical and cutting-edge techniques. These include plasmid manipulation, genetic complementation, mutagenesis, PCR, DNA sequencing, enzyme assays, and gene expression studies. Instruction and practice in written and oral communication are also emphasized. WARNING NOTICE The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such safety procedures and measures. MIT shall have no responsibility, liability, or risk for the content or implementation of any of the material presented.Legal Notice |
| 关 键 词: | 微生物学; 遗传学; 假单胞菌; 细菌; 基因; 病原体; PCR; DNA测序; 酶分析; 基因表达; 分子遗传学; 质粒操作; 遗传互补; 协议; 载体; 突变体; 囊性纤维化 |
| 课程来源: | 麻省理工学院公开课 |
| 最后编审: | 2017-11-26：cmh |
| 阅读次数: | 27 |