图 书 馆
基于序列的黑曲霉蛋白质分泌成功率预测Sequence-based Prediction of Protein Secretion Success in Aspergillus niger |
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| 课程网址: | http://videolectures.net/prib2010_vandenberg_spps/ |
| 主讲教师: | Bastiaan van den Berg |
| 开课单位: | 德尔夫特理工大学 |
| 开课时间: | 2010-10-14 |
| 课程语种: | 英语 |
| 中文简介: |  |
| 课程简介: | The cell-factory Aspergillus niger is widely used for industrial enzyme production. To select potential proteins for large-scale production, we developed a sequence-based classifier that predicts if an over-expressed homologous protein will successfully be produced and secreted. A dataset of 638 proteins was used to train and validate a classifier, using a 10-fold cross-validation protocol. Using a linear discriminant classifier, an average accuracy of 0.85 was achieved. Feature selection results indicate what features are mostly defining for successful protein production, which could be an interesting lead to couple sequence characteristics to biological processes involved in protein production and secretion. |
| 关 键 词: | 细胞工厂; 工业酶; 蛋白质 |
| 课程来源: | 视频讲座网 |
| 最后编审: | 2019-09-14：lxf |
| 阅读次数: | 44 |