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纳米接枝和自组装DNA单层杂交特性的原位比较
In situ comparison of hybridization properties of nanografted and self-assembled DNA monolayers |
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| 课程网址: |
http://videolectures.net/slonano07_grunwald_sch/
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| 主讲教师: |
Christian Grunwald
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| 开课单位: |
约瑟夫·斯特凡学院
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| 开课时间: |
2008-02-12
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| 课程语种: |
英语
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| 中文简介: |
使用常规的AFM和纳米图案的低密度和高密度单链DNA SAM，我们研究了在各种杂交时间和不同加载力下表面束缚的DNA分子的弹性响应。如所预期的，杂交后，仅对于低密度单链DNA SAM，可以看到向“直立”相的转变。我们发现，高密度DNA SAMs缺乏杂交的能力，不是因为密度高，而是因为固有的无序性，低DNA SAMs高度反映了这种固有性。已知纳米接枝会增加堆积密度和分子序。因此，我们已经在补丁实验中进行了补丁，以纳米图案化常规的低密度单链DNA SAM，并进行了2个后续的纳米移植过程。首先，创建HS C6 OH分子的参考区域，在第二个过程中，将单链DNA纳米移植到其中。因此，可以在常规DNA SAMs和纳米移植的DNA补丁之间的等效条件下进行精确的原位比较。并排的高度和压缩测量可以为接近固体界面的DNA分子的组织提供有价值的生物物理见解，例如：区分分子序和密度效应。
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| 课程简介: |
Using a conventional AFM and nano-patterned low and high density single stranded DNA SAMs we have investigated the elastic response of surface tethered DNA molecules after various hybridization times and under different loading forces. Upon hybridization, as expected, only for the low density single stranded DNA SAM a transition to the “standing up” phase is seen. We find that high density DNA SAMs are lacking the capability to hybridize not because of density but because of inherent disorder which is reflected by their low DNA SAM height. Nanografting is known to increase both packing density and molecular order. Therefore we have performed patch-in-a-patch experiments to nano-pattern a conventional low density single stranded DNA SAM with 2 subsequent nanografting processes. First a reference area of HS-C6-OH molecules was created into which in a second process single stranded DNA was nanografted. Thereby an accurate in situ comparison under equivalent conditions between conventional DNA SAMs and nanografted DNA patches becomes accessible. Side by side height and compression measurements can provide valuable biophysical insights on the organization of DNA molecules close to a solid interface e.g. discriminating between molecular order and density effects.
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| 关 键 词: |
纳米; 弹性响应; 杂交
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| 课程来源: |
视频讲座网
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| 最后编审: |
2019-09-25：yuh
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| 阅读次数: |
46
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