下一代测序技术在基因表达研究中的应用。我们到了吗?Application of Next Generation Sequencing technology to gene expression studies. Are we there yet? |
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课程网址: | http://videolectures.net/nib_nagy_sequencing/ |
主讲教师: | István Nagy |
开课单位: | Sekomics生物技术有限公司 |
开课时间: | 2011-10-11 |
课程语种: | 英语 |
中文简介: | 新的高通量深度测序技术极大地改变了研究基因组和转录体功能复杂性的方式。此外,SOLiD平台为监测给定样本的转录变化提供了两种可能:数字基因表达(DGE)分析和全转录测序(RNA-Seq)。Nagy I.等人。已经成功地使用了这两种方法;将讨论这些分析的结果以及QRT-PCR对结果的验证。 |
课程简介: | Novel high-throughput deep sequencing technology has dramatically changed the way that the functional complexity of genomes and transcriptomes can be studied. In addition, SOLiD platform provides two possibilities for monitoring the transcriptional changes of a given sample: Digital Gene Expression (DGE) analysis and full transcritpome sequencing (RNA-Seq). Nagy I. et al. have successfully used both approaches; the results of these analyses and validation of the findings by QRT-PCR will be discussed. |
关 键 词: | 测序; 数字基因表达; 高通量 |
课程来源: | 视频讲座网 |
数据采集: | 2020-11-16:yxd |
最后编审: | 2020-11-16:yxd |
阅读次数: | 27 |