图 书 馆
相关的肺泡体外模型能够可靠地研究纳米材料与细胞之间的相互作用A relevant in vitro model of the alveolus enables reliable studies of nanomaterial-cell interactions |
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| 课程网址: | http://videolectures.net/biophysics2018_majaron_vitro_model/ |
| 主讲教师: | Hana Majaron |
| 开课单位: | 约瑟夫·斯特凡学院 |
| 开课时间: | 2018-07-09 |
| 课程语种: | 英语 |
| 中文简介: |  尽管我们一直不断地暴露于空气传播的纳米材料，但我们仍无法预测其潜在的不良后果，这是由于我们对纳米材料与生物分子和细胞的基本相互作用缺乏了解而引起的一个问题。为了研究这些生物物理相互作用，我们设计了一个暴露于纳米材料的肺泡的简单体外模型，与广泛使用的浸入式体外模型相比，该模型与实际生理条件更为相似。这样，将导致更可靠地确定用于描述纳米材料毒性的相关分子相互作用。我们已经测试了将外源性肺表面活性剂应用于肺泡II型细胞的几种方法，并且发现就表面活性剂层的均匀性而言，肺表面活性剂的雾化优于移液。随后的高度浓缩的纳米粒子雾化使细胞均匀地暴露于纳米材料，从而无需使用与生理无关的蛋白质（主要是白蛋白）分散纳米材料。使用这两项改进，可以得到暴露于空气中纳米材料的简单的肺泡体外模型，从而可以使用超高分辨率STED显微镜实时研究相关系统上的生物物理相互作用。我还将介绍我们当前体外模型的缺点以及建议的未来升级。 |
| 课程简介: | Although we are constantly being exposed to airborne nanomaterial, we are still unable to predict its potential adverse outcomes - an issue arising from our lack of understanding of the basic interactions of nanomaterial with biomolecules and our cells. In order to study these biophysical interactions, we designed a simple in vitro model of the lung alveolus, exposed to nanomaterial, which would more closely resemble the actual physiological conditions than the widely-used submerged in vitro models. As such, it would result in more reliable determination of relevant molecular interactions for description of nanomaterial toxicity. We have tested several methods of application of exogenous lung surfactant onto alveolar type II cells, and have found nebulization of lung surfactant to be the superior to pipetting with regard to the homogeneity of the surfactant layer. The subsequent nebulization of highly-concentrated nanoparticles enables a uniform exposure of cells to nanomaterial eliminating the need to disperse nanomaterial using physiologically irrelevant proteins, mainly albumin. Using these two improvements results in a simple in vitro model of lung alveoli, exposed to airborne nanomaterial, which enables studying biophysical interactions on a relevant system in real-time using super-resolution STED microscopy. I will also present the drawbacks of our current in vitro model and some suggested future upgrades. |
| 关 键 词: | 纳米材料; 纳米材料毒性; 表面活性剂 |
| 课程来源: | 视频讲座网 |
| 数据采集: | 2020-11-22：cjy |
| 最后编审: | 2020-11-22：cjy |
| 阅读次数: | 30 |