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瞬态化学梯度中血细胞和小泡的扩散电泳Diffusiophoresis of blood cells and vesicles in transient chemical gradients |
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| 课程网址: | http://videolectures.net/biophysics2018_vrhovec_hartman_chemical_... |
| 主讲教师: | Saša Vrhovec Hartman |
| 开课单位: | 卢布尔雅那大学 |
| 开课时间: | 2018-07-09 |
| 课程语种: | 英语 |
| 中文简介: |  微腔提供了良好控制的无流动微环境，因此在许多微流体系统中起着重要作用，例如以细胞培养微腔的形式。在这里，我们显示了在微腔中溶质的扩散交换过程中出现的瞬时浓度梯度可以诱导血细胞和合成磷脂囊泡显着的被动迁移，这种作用被广泛地称为扩散电泳。我们观察到了具有相同渗透压的不同电解质溶液和非电解质溶液在交换过程中的迁移。根据溶液的类型，细胞和囊泡向腔的入口或末端迁移，迁移距离为十微米。因此，在生物医学和生物技术的许多应用中，观察到的现象会显着影响细胞行为。 |
| 课程简介: | Microcavities provide a well-controlled flow-free microenvironment and thus play an important role in many microfluidic systems, for example in the form of cell-culturing microchambers. Here we show that transient concentration gradients that emerge during diffusive exchange of solutes in microcavities can induce noticeable passive migration of blood cells and synthetic phospholipid vesicles, an effect that has been broadly termed as diffusiophoresis. We observed migration during the exchange of different electrolyte and non-electrolyte solutions that all had the same osmolarity. Depending on the type of solutions, cells and vesicles migrated towards the entrance or towards the end of the cavity, with migration distances on the order of ten micrometres. Thus, the observed phenomena can significantly affect cell behaviour in many applications of microfluidic devices in biomedicine and biotechnology. |
| 关 键 词: | 微腔; 无流动微环境; 扩散电泳 |
| 课程来源: | 视频讲座网 |
| 数据采集: | 2020-11-22：cjy |
| 最后编审: | 2020-11-22：cjy |
| 阅读次数: | 49 |