图 书 馆
充电接口上的单节电池Single cell at the charged interface |
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| 课程网址: | http://videolectures.net/biophysics2018_de_nardis_single_cell/ |
| 主讲教师: | Nadica Ivošević DeNardis |
| 开课单位: | 鲁尔·博斯科维奇学院 |
| 开课时间: | 2018-07-09 |
| 课程语种: | 英语 |
| 中文简介: |  单细胞电极相互作用与涉及细胞粘附，细胞融合，细胞活性和膜对环境压力的反应的基本生物学过程有关。我们的重点是作为全球重要的初级生产者，重要生物地球化学循环的驱动因素以及目前水生食物网的基础的单细胞海洋微藻。选定的藻类细胞仅具有细胞膜，具有明显的运动性，并能耐受多种盐度。使用互补表面方法和数学建模，将显示藻类细胞纳米机械性能及其生长过程中粘附动态的变化。原子力显微镜的测量表明，纳米机械性能会在细胞生长过程中发生变化，这反映在界面粘附动力学中。原子力显微镜的结果表明：（i）细胞比固定相的指数大，这与通过安培法测定的界面置换电荷量更大，并且（ii）指数相的细胞比那些固定的细胞更坚硬，更疏水在固定相中，这提示衰老过程中细胞被膜的分子修饰。通过三步过程（1）的反应动力学模型获得的结果表明，当细胞处于指数相时，在带电界面处的粘附和扩散较慢，这可能归因于更大的体积以及更坚硬和更具疏水性的细胞材料。将来，细胞的机械性能可以被认为是环境胁迫的标志，以便更好地了解水生界面处的藻类行为。 |
| 课程简介: | The single cell-electrode interaction is relevant to fundamental biological processes involving cell adhesion, cell fusion, cell activity, and membrane response to environmental stress. Our focus is on single-celled marine microalgae as the globally important primary producers, drivers of essential biogeochemical cycles and present the basis of the aquatic food web. Selected algal cell specie possesses only cell membrane, pronounced motility and tolerates a wide range of salinity. It will be shown changes of algal cell nanomechanical properties and adhesion dynamics during their growth using complementary surface methods and mathematical modelling. AFM measurements reveal that nanomechanical properties change during cell growth, and this is reflected in the interfacial adhesion dynamics. AFM results show that: (i) cells are larger in exponential than in the stationary phase, which is in an agreement with the amperometrically determined larger amount of interfacial displaced charge and (ii) cells in the exponential phase are stiffer and more hydrophobic than those in the stationary phase, which suggests molecular modification of cell envelope during aging. Results obtained with the reaction kinetics model of the three-step process (1) indicate that adhesion and spreading at the charged interface is slower when cells are in exponential phase, which may be attributed to larger volume and stiffer and more hydrophobic cellular material. In the future, cell mechanical properties could be considered as a marker for environmental stress in order to better understand algal behaviour at the aquatic interfaces. |
| 关 键 词: | 单细胞电极; 互补表面方法; 界面粘附动力学 |
| 课程来源: | 视频讲座网 |
| 数据采集: | 2020-11-23：cjy |
| 最后编审: | 2020-11-23：cjy |
| 阅读次数: | 42 |