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改进（神经）生物学的高分辨率光学成像Improving high-resolution optical imaging for (neuro-)biology |
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| 课程网址: | http://videolectures.net/biophysics2018_danzl_neuro_biology/ |
| 主讲教师: | Johann G. Danzl |
| 开课单位: | 奥地利科学技术研究所 |
| 开课时间: | 2018-07-09 |
| 课程语种: | 英语 |
| 中文简介: |  |
| 课程简介: | Far-field optical super-resolution microscopy or nanoscopy techniques “super-resolve” features residing closer than the diffraction-limit by transiently preparing fluorophores in distinguishable (typically on- and off-) states and reading them out sequentially. In coordinate-targeted super-resolution modalities, such as stimulated emission depletion (STED) microscopy, this state difference is created by patterns of light, driving for instance all molecules to the off-state except for those residing at intensity minima. I will discuss our recent efforts to improve coordinate-targeted nanoscopy. As a specific example, I will highlight how the use of multiple off-state transitions for nanoscopy can improve repeated imaging capability and on/off state contrast, enhancing image resolution and signal-to-background ratio in an approach that we dubbed “protected STED” (1). This allowed e.g. decoding the elaborate 3D structure of dendritic spines in living brain tissue. I will also give an outlook on the activities of my recently founded group at IST Austria. (1) Danzl, Sidenstein et al.. Coordinate-targeted fluorescence nanoscopy with multiple off-states. Nature Photonics 2016; 10: 122. |
| 关 键 词: | 远场光学超分辨显微镜; 纳米技术; 超分辨率模式 |
| 课程来源: | 视频讲座网 |
| 数据采集: | 2021-11-11：zkj |
| 最后编审: | 2021-11-11：zkj |
| 阅读次数: | 55 |