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星形细胞小泡的胞吐特性Exocytotic properties of astrocytic vesicles |
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| 课程网址: | http://videolectures.net/biophysics2018_jorgacevski_astrocytic_ve... |
| 主讲教师: | Jernej Jorgačevski |
| 开课单位: | 卢布尔雅那大学 |
| 开课时间: | 2018-07-09 |
| 课程语种: | 英语 |
| 中文简介: |  |
| 课程简介: | An important mode of intercellular communication involves the release of molecules from cells by exocytosis. Exocytotic transmitter release is most likely regulated by the SNARE complex, which contains a vesicular protein, synaptobrevin2 (Sb2). By using super-resolution stimulated emission depletion (STED) microscopy and structured illumination microscopy (SIM), we showed that in astrocytes smaller vesicles contain amino acid and peptidergic transmitters and larger vesicles contain ATP (1). We determined that an average astrocytic vesicle contains 15–25 endogenous Sb2 molecules (2). Then we monitored the interaction of a single vesicle with the plasma membrane by a high-resolution membrane capacitance approach. Astrocyte stimulation increases the frequency of predominantly transient fusion exocytotic events in smaller vesicles, whereas larger vesicles proceed more likely to full fusion exocytosis. Treatment of astrocytes with botulinum neurotoxins D and E, and dnSNARE peptide (coding the cytoplasmic domain of Sb2) stabilized the fusion-pore diameter to narrow, release-unproductive diameters in both vesicle types, regardless of vesicle diameter (1). Astrocytes also exhibit strong vesicle related phenotype in certain disorders, like X-linked non-syndromic intellectual disability (XLID), that have traditionally been linked to neurons. |
| 关 键 词: | 细胞间通讯; 胞外递质释放; 星形细胞囊泡 |
| 课程来源: | 视频讲座网 |
| 数据采集: | 2021-11-11：zkj |
| 最后编审: | 2021-11-11：zkj |
| 阅读次数: | 61 |