一般麻醉剂在模型膜中的位置Location of the general anesthtics in model membranes |
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课程网址: | http://videolectures.net/biophysics2018_hrubovcak_model_membranes... |
主讲教师: | Pavol Hrubovcak |
开课单位: | 联合核研究所 |
开课时间: | 2018-07-09 |
课程语种: | 英语 |
中文简介: | 初级脂肪醇和烷烃的麻醉作用是众所周知的,但其来源问题尚未得到充分回答。由于生物膜结构的变化与其特定功能之间存在着非常密切的关系,因此有一种假说认为,脂肪族分子与细胞膜成分的相互作用产生了麻醉作用。一个掺杂正癸烷的双层体系的案例研究提出了该模型,该模型假设正癸烷分子在双层中的位置和方向取决于其浓度。根据该模型,正癸烷分子最有可能选择两种可能的位置之一。它们要么集中在与其两亲链平行的脂质分子中,要么位于双层的中心,垂直于脂质链。该假设的缺点是对实验数据的解释模棱两可。为了进一步阐明这个问题,我们设计并进行了小角度中子衍射实验。我们制备了含有氘标记和未标记正癸烷分子的模型磷脂(DOPC)膜。利用对比度变化法和中子衍射数据的相互比较,我们能够确定正癸烷分子在膜内的位置。 融资:维加赠款1/0916/16;JINR项目04-4-1121-2015/2017;ILL通过中欧中子倡议 |
课程简介: | The anesthetic effect of primary aliphatic alcohols and alkanes is known generally, however, the question of its origin has not been sufficiently answered yet. Since there is a very close relation between changes in a structure of biomembrane and its specific functions, there is a hypothesis, which suggests the anesthetic effect stemming from the interaction of aliphatic molecules with cellular membrane constituents. A case study of bilayer systems doped with n-decane put forward the model, which assumes that the location and orientation of n-decane molecules within bilayer depends on their concentration. According to the model, molecules of n-decane most likely prefer one of the two possible positions. Either they concentrate among the lipid molecules with parallel orientation to their amphiphilic chains or they are located in the center of the bilayer, perpendicular to the lipid chains. The drawback of the hypothesis is in the ambiguous interpretation of experimental data. In order to shed more light on this question, we designed and performed a small-angle neutron diffraction experiment. We prepared model phospholipid (DOPC) membranes containing deuterium-labelled and unlabeled molecules of n-decane. Employing contrast variation method and mutual comparison of neutron diffraction data obtained for labeled and unlabeled samples, we were able to determine the location of n-decane molecules within the membrane. |
关 键 词: | 麻醉; 小角度中子衍射实验; 正癸烷分子 |
课程来源: | 视频讲座网 |
数据采集: | 2021-11-13:zkj |
最后编审: | 2021-11-13:zkj |
阅读次数: | 55 |