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第四讲-基因工程（续）Lecture 4 - Genetic Engineering (cont.) |
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| 课程网址: | http://videolectures.net/yalebeng100s08_saltzman_lec04/ |
| 主讲教师: | W. Mark Saltzman |
| 开课单位: | 耶鲁大学 |
| 开课时间: | 2011-01-04 |
| 课程语种: | 英语 |
| 中文简介: |  |
| 课程简介: | Professor Saltzman continues his presentation on DNA technology by discussing control of gene expression using two methods of RNA silencing: anti-sense therapy and RNA interference. Molecular cloning techniques to mass-produce proteins using plasmid, restriction enzymes, ligase, and antibiotic selection in bacteria are discussed. Steps and molecules involved in polymerase chain reaction are also described. Professor Saltzman explains how to detect mutations in genomic DNA, such as in sickle cell anemia patients, by gel electrophoresis and Southern blotting. Finally, he gives an example of inducing and controlling insulin expression in large animals by cloning into their genome the insulin gene with the lac promoter. |
| 关 键 词: | DNA技术; 控制基因表达; 反义疗法 |
| 课程来源: | 视频讲座网 |
| 数据采集: | 2021-11-27：zkj |
| 最后编审: | 2021-11-27：zkj |
| 阅读次数: | 96 |