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应激通过瘦素影响Sprague-Dawley大鼠肾上腺干细胞的存活
Stress affects survival of adrenal gland stem cells through leptin in Sprague Dawley rats |
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| 课程网址: |
http://videolectures.net/snc2015_balog_stress_affects/
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| 主讲教师: |
Marta Balog
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| 开课单位: |
奥西耶克尤拉伊大学
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| 开课时间: |
2015-10-01
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| 课程语种: |
英语
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| 中文简介: |
在现代社会，压力是每天都要面对的挑战。它强烈参与下丘脑-垂体-肾上腺轴。急性压力促进“战斗或逃跑”反应，而慢性压力导致多种疾病。瘦素是影响肾上腺的重要激素，肾上腺是应激反应的主要器官。在本研究中，我们假设瘦素通过其受体在肾上腺干细胞存活中发挥重要作用。研究中使用了22只雄性、24只雌性（非卵子）和24只4个月大的Sprague-Dawley大鼠的去卵巢雌性（卵子）。动物进一步分为对照组、急性和慢性应激组。用瘦素受体（Ob-R）一级抗体和二级FITC结合荧光抗体对7月龄大鼠肾上腺进行免疫染色。图像由徕卡TCSSP8共聚焦显微镜拍摄，并通过ImageJ软件进行分析。我们注意到肾上腺干细胞层的免疫染色。非OVX对照组的免疫染色强度明显高于OVX对照组（F=6.2；p<0.05）。在急性应激时，雄性大鼠的荧光强度高于非OVX大鼠（F=6.56；p<0.05）。慢性应激组间无统计学意义，但各组免疫染色强度均强于急性应激组。免疫染色强度在动物组内的重要统计意义-雄性（χ2（2）=12.31；p=0.002），非OVX（χ2（2）=12.11；p=0.002）和OVX（χ2（2）=15.15；p=0.0005）。瘦素可能参与女性体内平衡和一般应激调节。由于瘦素调节葡萄糖摄取并抑制应激诱导的细胞凋亡，这一发现可能表明瘦素在干细胞存活中的重要作用以及对肾上腺应激反应的长期影响。
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| 课程简介: |
Stress is an everyday challenge in the modern world. It is strongly engaging the hypothalamic-pituitary-adrenal axis. Acute stress promotes ˝fight-or-flight˝ response while chronic stress leads to a variety of diseases. Leptin is an important hormone affecting adrenal gland, the chief organ of the stress response. In this study we hypothesize that leptin plays an important role in adrenal gland stem cells survival through its receptor. 22 male, 24 female (NON-OVX) and 24 ovariectomized female (OVX) of 4-months-old Sprague Dawley rats were used in the study. Animals were further divided in control, acute and chronic stress groups. Adrenal glands of 7-months-old rats were immunostained with leptin receptor (Ob-R) primary antibody and secondary FITC-conjugated fluorescent antibody. Images were photographed by Leica TCSSP8 confocal microscope and analyzed by ImageJ software. We noticed immunostaining in the stem cells layer of adrenal gland. NON-OVX control group had statistically higher immunostaining intensity than OVX control group (F = 6.2; p < 0.05). Males had statistically higher fluorescence intensity than NON-OVX upon acute stress (F = 6.56; p < 0.05). Chronic stress did not produce statistical significances among groups, but immunostaining intensity was stronger in all groups than upon acute stress. Important statistical significance in immunostaining intensity occured inside animal groups – males (χ2(2) = 12.31; p = 0.002), NON-OVX (χ2(2) = 12.11; p = 0.002) and OVX (χ2(2) = 15.15; p = 0.0005). Leptin might be involved in homeostasis in females and in general stress regulation. Since leptin regulates glucose uptake and inhibits stress-induced apoptosis this finding might indicate its important role in stem cells survival and long-term affect on stress response by adrenal gland.
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| 关 键 词: |
压力; 瘦素; 肾上腺
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| 课程来源: |
视频讲座网
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| 数据采集: |
2021-12-14：nkq
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| 最后编审: |
2021-12-14：nkq
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| 阅读次数: |
38
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