图 书 馆
小RNA测序和组装：从防御分子重建病毒基因组Small RNA sequencing and assembly: reconstructing viral genomes from defence molecules |
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| 课程网址: | http://videolectures.net/nib_kreuze_sequencing/ |
| 主讲教师: | Jan Kreuze |
| 开课单位: | 国际马铃薯研究中心 |
| 开课时间: | 2013-05-07 |
| 课程语种: | 英语 |
| 中文简介: |  |
| 课程简介: | RNA silencing constitutes a fundamental antiviral defence mechanism in plants in which host enzymes cut viral RNA into pieces of 20-24 nucleotides. When isolated, sequenced en mass and properly assembled or aligned these virus-derived small RNA (sRNA) sequences can reconstitute genomic sequence information of the viruses being targeted in the plant. This approach is independent of the ability to culture or purify the virus and does not require any specific amplification or enrichment of viral nucleic acids as it automatically enriches for small RNAs of viral origin by tapping into a natural antiviral defence mechanism. Results from the application of this technique to identify novel plant viruses as well as map variability and distribution of viruses will be presented. |
| 关 键 词: | RNA沉默; 植物抗病毒; 基本防御机制 |
| 课程来源: | 视频讲座网 |
| 数据采集: | 2022-03-26：zkj |
| 最后编审: | 2022-03-26：zkj |
| 阅读次数: | 61 |