图 书 馆
两种植物和病原菌之间的分子对话Molecular dialog between two ingenious opponents-plants and pathogenic bacteria |
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| 课程网址: | http://videolectures.net/nib_krzymowska_pathogenic_bacteria/ |
| 主讲教师: | Magdalena Krzymowska |
| 开课单位: | 波兰科学院生物化学和生物物理学研究所 |
| 开课时间: | 2014-02-24 |
| 课程语种: | 英语 |
| 中文简介: |  |
| 课程简介: | Pseudomonas syringae pv. phaseolicola is the causative agent of halo blight in Phaseolus vulgaris L., the common bean. Similar to other pathogenic Gram-negative bacteria, P. syringae delivers type III virulence factors into host cells. These factors are subject to the competing forces of improved pathogenesis and avoidance of recognition by the host. This concept is particularly well illustrated by HopQ1 (for Hrp outer protein Q), which functions to promote disease development in the bean and tomato. However, HopQ1 is sensed by Nicotiana spp., which has evolved systems to respond to its presence. The mechanistic explanation for this phenomenon has not yet been provided. We found that HopQ1, after specific phosphorylation, associates with host 14-3-3 proteins, a highly-conserved family of proteins that function as regulators of their partner proteins. We confirmed the physical interaction between HopQ1 and 14‐3‐3a using FRET‐FLIM techniques. A mutation that prevented binding between 14-3-3 and HopQ1 proteins affected the cellular distribution and stability of the effector and slightly compromised bacterial growth in the bean. Interestingly, tobacco plant recognition of HopQ1 was not altered by that mutation, which suggests the presence of separate virulence and avirulence determinants. We are currently analyzing structures of monomeric and oligomeric forms of HopQ1 and the complex of HopQ1 with 14-3-3a, using MALS (multiangle light scattering analysis) combined with size exclusion chromatography and SAXS (small angle X-ray scattering) techniques supported by molecular modeling. Results so far will be presented. |
| 关 键 词: | 病原菌; 单胞菌; 宿主细胞 |
| 课程来源: | 视频讲座网 |
| 数据采集: | 2022-03-27：zkj |
| 最后编审: | 2022-04-08：zkj |
| 阅读次数: | 65 |