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利用纳米孔进行单分子酶学和蛋白质测序Harnessing nanopores for single-molecule enzymology and protein sequencing |
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| 课程网址: | http://videolectures.net/preglov_maglia_harnessing_nanopores/ |
| 主讲教师: | Giovanni Maglia |
| 开课单位: | 格罗宁根大学 |
| 开课时间: | 2018-09-25 |
| 课程语种: | 英语 |
| 中文简介: |  |
| 课程简介: | Biological nanopores are a class of membrane proteins that form nanometer-size apertures on lipid membranes. Under an applied potential the ionic current through nanopores is used to identify molecules or to follow reactions at the single-molecule level. Nanopores current are advantageous because they are easily integrated into low-cost and portable analytical devices. Here we show that the transport of proteins and peptides across nanopores can be finely controlled. Folded proteins can trapped inside nanopores by exploiting electrophoretic forces, while small peptides can be stretched and unfolded by engineering strong electroosmotic flows through the nanopore. Using these approaches, nanopores may be used as nanoscale reactor for single-molecule enzymology studies, or sensors for sequencing individual proteins as they translocate across the nanopore. |
| 关 键 词: | 纳米孔; 单分子酶学; 蛋白质测序 |
| 课程来源: | 视频讲座网 |
| 数据采集: | 2020-07-14：csy |
| 最后编审: | 2021-09-20：zyk |
| 阅读次数: | 64 |