图 书 馆
分子生物学方法Methods in Molecular Biology |
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| 课程网址: | https://ocw.vu.edu.pk/CourseDetails.aspx?cat=Biotechnology&course... |
| 主讲教师: | Dr. Saeeda Kalsoom |
| 开课单位: | 巴基斯坦虚拟大学 |
| 开课时间: | 信息不详。欢迎您在右侧留言补充。 |
| 课程语种: | 英语 |
| 中文简介: |  聚合酶链式反应，PCR步骤，PCR成分，PCR引物，DNA聚合酶，标准PCR反应，结果解释，PCR类型，嵌套PCR，多重PCR，逆转录酶PCR，实时PCR，热启动PCR，不对称PCR，长PCR，等位基因特异性PCR，菌落PCR，原位PCR，反向PCR，AFLP PCR，组装PCR，自杀PCR，甲基化特异性PCR，序列间特异性PCR，连接介导的PCR，全基因组扩增PCR，迷你引物PCR，PCR的优缺点，PCR的应用，PCR医学，PCR法医科学，PCR农业科学与环境，PCR分子古生物学，限制和修饰（RM）系统的类型，限制核酸内切酶的命名法，限制核酸内切酶的靶位点，限制性片段的数量和大小，限制性核酸内切酶概述，DNA修饰酶，连接DNA片段的方法，DNA连接酶产生共价重组DNA，碱性磷酸酶酶，通过接头分子的平末端连接，衔接子，Southern印迹，Southern印迹程序，Southern印迹探针，Southern印迹的转移方法，Southern印迹鉴定突变，Northern印迹，Northern印迹应用，Western印迹，Western印迹程序 |
| 课程简介: | Polymerase chain reaction, Steps of PCR, Ingredients of PCR, Primers for PCR, DNA polymerases, Standard PCR reaction, Interpretation of results, Types of PCR, Nested PCR, Multiplex PCR, Reverse transcriptase PCR, Real time PCR, Hot start PCR, Asymmetric PCR, Long PCR, Allelic specific PCR , Colony PCR, In situ PCR, Inverse PCR, AFLP PCR, Assembly PCR, Suicide PCR, Methylation specific PCR, Inter sequence specific PCR, Ligation mediated PCR, Whole genome amplification PCR, Mini primer PCR, Advantages and limitations of PCR, Applications of PCR, PCR-Medicine, PCR-Forensic sciences, PCR-Agricultural sciences and environment, PCR-Molecular paleontology, Types of restriction and modification (R-M) system, Nomenclature of restriction endonucleases ,Target sites of restriction endonucleases, Number and size of restriction fragments, Summary of restriction endonucleases, DNA modifying enzymes, Methods of joining DNA fragments, DNA ligase to create covalent recombinant DNA, Alkaline Phosphatase, Blunt end ligation via linker molecules, Adaptors, Southern blotting, Southern blotting procedure, Southern blotting probes, Transfer methods in southern blotting, Mutations identification by southern blotting, Northern blotting, Northern blot applications, Western blotting, Western blotting procedure |
| 关 键 词: | 聚合酶; PCR; 生物学 |
| 课程来源: | 巴基斯坦虚拟大学公开课 |
| 数据采集: | 2021-05-08：nkq |
| 最后编审: | 2021-05-08：nkq |
| 阅读次数: | 89 |