图 书 馆
微生物的质谱鉴定Identification of microorganisms by mass spectrometry |
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| 课程网址: | http://videolectures.net/biophysics2018_cindric_mass_spectometry/ |
| 主讲教师: | Mario Cindrić |
| 开课单位: | Ruđer Bošković研究所 |
| 开课时间: | 2018-07-09 |
| 课程语种: | 英语 |
| 中文简介: |  目前用于物种鉴定的技术主要依赖于蛋白质数据库匹配或免疫程序。尽管被广泛使用，但这种方法并不总能提供令人满意的匹配、序列覆盖或特异性抗原抗体反应，以明确识别所选物种的 DNA、RNA、脂质和糖或肽/蛋白质。大约十年前，质谱法作为物种确定的工具被引入。基质辅助激光解吸电离飞行时间质谱 (MALDI TOF MS) 使用完整细胞或细胞提取物识别微生物。仪器中检测到的细胞中最丰富的蛋白质与涵盖相对有限数量的微生物的市售数据库相匹配。另一方面，与 MALDI TOF MS 相比，高分辨率质谱仪可以在分析蛋白质的 de novo 测序后提供更准确和精确的结果。 |
| 课程简介: | Currently used techniques for species identification are mostly relied on protein database matching or alternatively on immunological procedures. Although widely used, such an approach does not always provide satisfactory matching, sequence coverage or specific antigen-antibody reaction to unambiguously identify DNA, RNA, lipid, and sugar or peptide/protein of selected species. Mass spectrometry as a tool for species determination was introduced about ten years ago. Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) identifies microbes using either intact cells or cell extracts. The most abounded proteins from the cell detected in the instrument are matched against commercially available databases that cover relatively limited number of microbes. On the other hand, high resolution mass spectrometers in contrast to MALDI-TOF MS could provide more accurate and precise results after de novo sequencing of analyzed proteins. |
| 关 键 词: | 物种鉴定; 质谱法; 免疫程序 |
| 课程来源: | 视频讲座网 |
| 数据采集: | 2021-07-05：liyy |
| 最后编审: | 2021-07-06：liyy |
| 阅读次数: | 73 |