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斯洛文尼亚母子对中金属硫蛋白多态性和微量元素Metallothionein polymorphisms and trace elements in Slovenian mother-child pairs (CROME-LIFE+ and HEALS study) |
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| 课程网址: | http://videolectures.net/ipssc2017_stajnko_metallothionein_polymo... |
| 主讲教师: | Anja Stajnko |
| 开课单位: | 约瑟夫·斯特凡研究所 |
| 开课时间: | 2017-05-23 |
| 课程语种: | 英语 |
| 中文简介: |  |
| 课程简介: | Methallothioneins (MT) are metal(loids) binding proteins characterised by low molecular weight (6-7 kDa), high cysteine content (30%) and high metal affinity [1]. They have several roles in physiological system, especially in metal(loid)s homeostasis and detoxification and cellular oxidative stress protection [1,2]. Human methallothioneins are coded by at least 11 functionally active genes that are located on chromosome 16 and single nucleotide polymorphisms (SNPs) in these genes, could modify proteins functions [3,4]. In present study the data from 178 non-occupationally exposed Slovenian mother-child pairs (mothers: mean age = 38.6 years; child: 7-8 years, 50% females, 2016) was used to estimate possible associations between genotypes of various selected SNPs in MT genes (MT1a, MT1b, MT1e, MT1f, MT1g, MT1x; MT2a; MT3; MT4) and trace elements (Hg, As, Pb, Cd, Zn, Cu, Mn, Se). DNA was extracted from venous blood and used for SNP genotypisation by pre-designed TaqMan® assays (Applied Biosystems, USA) and quantitative real time PCR (qPCR), while the concentrations of trace elements were determined in blood and urine samples using an inductive-coupled plasma mass spectrometry (ICP-MS). Obtained significant associations between genotypes and trace elements were further tested by multiple linear regression models for possible confounders (age, gender, body mass index, education, current or past smoking, food consumption, essential element status etc.). |
| 关 键 词: | 甲硫蛋白; 离子体质谱; 基因型和微量元素 |
| 课程来源: | 视频讲座网 |
| 数据采集: | 2021-11-11：zkj |
| 最后编审: | 2021-11-11：zkj |
| 阅读次数: | 60 |