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双层脂质膜作为肿瘤细胞光动力治疗过程的模型
Bilayer lipid membrane as a model of photodynamic therapy processes in cancer cells |
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| 课程网址: |
http://videolectures.net/biophysics2018_munguia_lipid_membrane/
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| 主讲教师: |
Irene Jimenez Munguia
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| 开课单位: |
国立科学技术大学
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| 开课时间: |
2018-07-09
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| 课程语种: |
英语
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| 中文简介: |
癌症的光动力疗法是基于使用光敏剂（PS）结合细胞，并通过光照下产生的单线态氧（SO）杀死细胞。这些过程在模型系统——双层脂膜（BLM）上进行了体外研究。我们研究了各种卟啉衍生物的吸附和光动力效率。PS与BLM的结合以及与之结合的特殊分子（SO靶）的损伤通过测量边界电位（BP）进行监测，该方法由我们先前开发。磺化四苯基卟啉（TPPS4）的阴离子为负，b-咪唑基取代四苯基卟啉（b-ImTPP）和相应的锌（II）络合物（b-ImTPPZn）的阳离子为正。在光照下，这些PS的光动力活性被评估为吸附在BLM表面的di-4-ANEPPS的SO目标（T）分子的损伤率R。将吸附在PS分子膜（顺）侧的T分子的氧化速率与吸附在反（反）侧的T分子的氧化速率进行了比较。当磺化铝（III）酞菁（1,2）生成SO时，顺式侧的R值高于反式侧的R值。卟啉和酞菁的区别可以通过这些PS在膜边界上的不同位置来解释。
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| 课程简介: |
The photodynamic therapy of cancer is based on using of photosensitizers (PS) which bind to cells and kill them by singlet oxygen (SO) generated under illumination. These processes were studied in vitro on a model system – bilayer lipid membrane (BLM). We have studied adsorption and photodynamic efficiency of various derivates of porphyrins. The binding of PS to BLM as well as damage of incorporated into it special molecules — SO targets — were monitored by measuring of boundary potential (BP) by a method developed by us earlier. The BP was negative in the case of anions of sulfonated tetraphenylporphyrines (TPPS4) and positive one with cations of b-imidazolyl substituted tetraphenylporphyrine (b-ImTPP) and corresponding Zn(II) complex (b-ImTPPZn). The photodynamic activity of these PS was evaluated as rate R of damage of the SO targets (T) — molecules of di-4-ANEPPS adsorbed on the surface of BLM, under illumination. The rate of oxidation of T molecules adsorbed on (cis) side of the membrane containing PS molecules was compared with that when T were adsorbed on opposite (trans) side. The value of R at cis- side was higher than that at trans- side in contrast to our earlier study when SO was generated by sulfonated aluminum(III) phthalocyanines (1,2). The difference between porphyrins and phthalocyanines was explained by different positions of these PS on the boundary of the membrane.
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| 关 键 词: |
光动力疗法; 双层脂膜; 卟啉衍生物
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| 课程来源: |
视频讲座网
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| 数据采集: |
2021-11-13：zkj
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| 最后编审: |
2021-11-13：zkj
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| 阅读次数: |
55
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