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肝素阻断胶质瘤细胞外小泡的摄取
Heparin blocks uptake of glioma-derived extracellular vesicles |
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| 课程网址: |
http://videolectures.net/nib_atai_heparin_blocks/
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| 主讲教师: |
Nadia A. Atai
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| 开课单位: |
学术医疗中心
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| 开课时间: |
2014-05-14
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| 课程语种: |
英语
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| 中文简介: |
细胞外小泡（EV）与肿瘤发生有关。能够阻止EV结合和吸收到受体细胞的生物分子可能具有治疗价值。在这里，我们显示肝素与肿瘤来源的EV进入受体细胞的摄取相互作用。将胶质瘤细胞衍生的EV与肝素孵育后，通过透射电子显微镜（TEM）观察到微米大小的结构，EV在这些结构中清晰可见。肝素的加入大大减少了标记EV从供体到受体肿瘤细胞的转移。我们还通过结合分析和显微镜观察显示了肝素和EVs之间的直接相互作用。我们发现EVs摄取的阻滞是在细胞结合水平，而不是内化水平。最后，含有EGFRvIII mRNA的胶质瘤衍生EV与肝素孵育可减少该信息向受体细胞的传递。肝素对EVs摄取的影响可能为研究EV功能提供一个独特的工具。它还可能促进肝素作为EV发挥作用的疾病治疗方法的研究。
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| 课程简介: |
Extracellular vesicles (EVs) have been implicated in tumorigenesis. Biomolecules which can block EV binding and uptake into recipient cells may be of therapeutic value. Here, we show that heparin interacts with uptake of tumor-derived EVs into recipient cells. Incubation of glioma cell-derived EVs with heparin resulted in micron-sized structures observed by transmission electron microscopy (TEM), with EVs clearly visible within these structures. Inclusion of heparin greatly diminished transfer of labeled EVs from donor to recipient tumor cells. We also show a direct interaction between heparin and EVs using binding assays and microscopy. We found that the block in EVs uptake was at the level of cell binding and not internalization. Finally, incubation of glioma-derived EVs containing EGFRvIII mRNA with heparin reduced transfer of this message to recipient cells. The effect of heparin on EVs uptake may provide a unique tool to study EV function. It may also foster research of heparin as a therapeutic for disease in which EVs play a role.
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| 关 键 词: |
细胞外小泡; 肿瘤; 肝素
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| 课程来源: |
视频讲座网
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| 数据采集: |
2021-12-17：zkj
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| 最后编审: |
2021-12-17：zkj
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| 阅读次数: |
50
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